We narrowed to 8,490 results for: tet on

1. pcDNA6 asyn E46K

   Plasmid

   #105730

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E46K

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRRLNeo-pEF1a-p53ash-mCerulean

   Plasmid

   #69579

   Purpose

   Expresses p53 tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQCXIP mScarlet-MOSPD2 WT

   Plasmid

   #186472

   Purpose

   Expression of human MOSPD2 fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

   Plasmid

   #69583

   Purpose

   Expresses p53 tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEGFP-PI35P2

   Plasmid

   #92419

   Purpose

   Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

   Insert

   MCOLN1 (Mcoln1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Synthetic gene, codon optimized for human.

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-IKKbeta (S177E,S181E)-pcw107

   Plasmid

   #64609

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   IKBKB
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S177E, S181E

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pScalps_Puro_mCebpa

   Plasmid

   #79551

   Purpose

   Expression of mouse Cebpa

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Cebp alpha (Cebpa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. myr-FLAG-PIK3CA-pcw107

    Plasmid

    #64605

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    PIK3CA (PIK3CA Bovine)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Expression

    Mutation

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pXL002-ishRNA-beta-catenin-1

    Plasmid

    #36297

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-1 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLenti4/TO-hEPO

    Plasmid

    #50437

    Purpose

    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

    Insert

    EPO (EPO Human)
    

    Use

    Lentiviral; Tetracycline-regulatable (t-rex)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid

    #193764

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔCy-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid

    #183765

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    ChR2-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXL003-ishRNA-beta-catenin-2

    Plasmid

    #36299

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-2 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Human SHIP1 (clone hp51CN)

    Plasmid

    #123347

    Purpose

    Expression of human SHIP1

    Depositor

    Christophe Erneux

    Article

    Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

    Insert

    INPP5D (INPP5D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H6GB1tevP53(1-303)IntN

    Plasmid

    #200312

    Purpose

    Bacterial expression of P53 NTAD-DBD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6GB1tev and designed intein N

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge