Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,343 results for: pcDNA

Showing: 5581 - 5600 of 12343 results

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pcDNA4/TO-2xStrep-UL32 C544A

Plasmid

#162647

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C544 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 H581A

Plasmid

#162648

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H581 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C155A

Plasmid

#162649

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C155 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 C52A

Plasmid

#162630

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 C373A

Plasmid

#162631

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C373 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA Lys162Met

Plasmid

#157752

Purpose

Expression of kinase-dead AURKA K62M in mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA 1-205 USP7

Plasmid

#131254

Purpose

Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575_AG

Plasmid

#148752

Purpose

Mammalian Expression of HsNot4_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575 (CNOT4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_1-274_AG

Plasmid

#148750

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM4 (G470A/G471N)

Plasmid

#154859

Purpose

Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM4 (PF3D7_0506900 Synthetic)

Tags

triple HA

Expression

Mammalian

Mutation

Glycine 470 changed to Alanine and Glycine 471 ch…

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM1 (A144G/N145G)

Plasmid

#154873

Purpose

Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM1 (PF3D7_0506900 Synthetic)

Tags

triple HA

Expression

Mammalian

Mutation

Alanine 144 changed to Glycine and Asparagine 145…

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-201-2xStrep

Plasmid

#138442

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-201

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-226-2xStrep

Plasmid

#138443

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-226

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-181-2xStrep

Plasmid

#138444

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-181

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-191-2xStrep

Plasmid

#138445

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-191

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-216-2xStrep

Plasmid

#138446

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-216

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 1-168-2xStrep

Plasmid

#138447

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-168

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 1-178-2xStrep

Plasmid

#138448

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-178

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5581 - 5600 of 12343 results

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