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3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Rab5c cds in pGem-T Easy

Plasmid

#80518

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5c (rab5c Zebrafish)

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Rab5b cds in pGem-T Easy

Plasmid

#80517

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5b (rab5b Zebrafish)

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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SP_gRNA_pUC19_N_FancF_Left

Plasmid

#79892

Purpose

Human gRNA expression vector targeting FANCF (site 1 left)

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

FancF_Site_1_Left

Use

CRISPR

Tags

None

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmPAN2

Plasmid

#79256

Purpose

Expresses EGFP-tagged DmPAN2 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-Y16C

Plasmid

#30372

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y16C (PTEN Human)

Expression

Mammalian

Mutation

Y16C

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
CD4_LD 0_TEV

Plasmid

#58870

Purpose

MESA protease chain with CD4 ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with CD4 ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pPZP FLIPmal-25u

Plasmid

#14476

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPmal

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

W230A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
miR-141 reporter

Plasmid

#67632

Purpose

To quantify miR-141 function

Depositor

Judy Lieberman

Article

Le et al J Clin Invest. 2014 Dec;124(12):5109-28.

Insert

2 binding sites for miR-141

Use

Luciferase

Promoter

SV40

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pmECFPC1/GgVcl 1-1066 (CFP at N term)

Plasmid

#46281

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

Vcl 1-1066 (VCL Chicken)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1/HisMax-Syx1

Plasmid

#35708

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

6x HIS and Xpress

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
2xDHFR-myc

Plasmid

#20215

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-FLAG-Nv-NF-kB-Cys

Plasmid

#27260

Depositor

Thomas Gilmore

Article

Sullivan et al PLoS One. 2009 . 4(10):e7311.

Insert

Nv-NF-kB

Tags

FLAG

Expression

Mammalian

Mutation

"Cys allele" = Cysteine at aa 67

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pSG5-p85α-ΔiSH2-N

Plasmid

#39498

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T H42Q

Plasmid

#9065

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only