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ISA861: Melezitose pathway

Plasmid

#241971

Purpose

Melezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Publication

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

melezitose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Publication

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-C-term_F

Plasmid

#146301

Purpose

Mammalian Expression of HsTNRC6Biso1-Cterm

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-Cterm (TNRC6B Human)

Expression

Mammalian

Mutation

Contains 7x Gln tract rather than 8x Gln tract

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsAGO1 (AGO1 Human)

Expression

Mammalian

Mutation

N574S

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO2_F

Plasmid

#146315

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO2 (AGO2 Human)

Expression

Mammalian

Mutation

one silent mutation C840T and sequence differs in…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pIT5_KP

Plasmid

#173774

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KO

Plasmid

#173775

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KW

Plasmid

#173776

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KD

Plasmid

#173771

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KL

Plasmid

#173770

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_C

Plasmid

#146086

Purpose

Mammalian Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso3_C

Plasmid

#146088

Purpose

Mammalian Expression of HsTNRC6Biso3

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HsTNRC6Biso3 (TNRC6B Human)

Expression

Mammalian

Mutation

AA 3-188 and AA residues 242-1029 (with N242D) co…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-MBP_F

Plasmid

#146318

Purpose

Mammalian Expression of MBP

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

MBP (MBP Human)

Expression

Mammalian

Mutation

N217S

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Publication

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

b2AR (ADRB2 Human)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-Olfr644

Plasmid

#149365

Purpose

Expresses Olfr644 in mammalian cells.

Depositor

Takeshi Imai

Publication

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Olfr644 (Olfr644 Mouse)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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cpi-17

Plasmid

#78708

Purpose

Encodes zebrafish cpi-17

Depositor

Sarah Childs

Publication

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

cpi-17 (ppp1r14aa Zebrafish)

Expression

Bacterial

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
nm-mhc-b

Plasmid

#78709

Purpose

Encodes zebrafish nm-mhc-b

Depositor

Sarah Childs

Publication

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

nm-mhc-b (myh10 Zebrafish)

Expression

Bacterial

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pTac-A-luci

Plasmid

#65933

Purpose

Theophylline Riboswitch A with luciferase gene

Depositor

Justin Gallivan

Publication

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Promoter

Tac

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only