We narrowed to 6,853 results for: addgene/1000

1. pDONR223_CDKN1B_WT

   Plasmid

   #81836

   Purpose

   Gateway Donor vector containing CDKN1B ,part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDKN1B (CDKN1B Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   V109G

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT3-ecdCD86-ST3-His

   Plasmid

   #210664

   Purpose

   Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
   

   Use

   Tags

   Histag and Spytag003

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-CD80-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156625

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD80 (CD80 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EGFR-L747_T751delLREAT

   Plasmid

   #116270

   Purpose

   Lentiviral expression of EGFR L747_T751delLREAT

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L747_T751delLREAT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-AMIGO3-COMP5AP-AviTag-9xHis

   Plasmid

   #157369

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   AMIGO3 (AMIGO3 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EGFR-R222C

   Plasmid

   #116293

   Purpose

   Lentiviral expression of EGFR R222C

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R222C

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-2xFLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172622

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPICZc-Ub-R-HsACTB*-thymosinB-8His

   Plasmid

   #111149

   Purpose

   Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   ACTB (ACTB Human)
   

   Use

   Pichia pastoris integration plasmid

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   N-terminal arginylation

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_GPR137B_WT

   Plasmid

   #82890

   Purpose

   Gateway Donor vector containing GPR137B, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   GPR137B (GPR137B Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-CD80-COMP5AP-AviTag-9xHis

    Plasmid

    #157189

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIG-709pUC19-tNGFR-P2A-IL10

    Plasmid

    #186117

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL10 (IL10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCB268

    Plasmid

    #53363

    Purpose

    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

    Depositor

    Alexander Varshavsky

    Article

    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

    Inserts

    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    

    Use

    Tags

    13xMyc, Flag, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

15. NGFR 210Y177

    Plasmid

    #27487

    Depositor

    Warren Pear

    Article

    He et al Blood. 2002 Apr 15;99(8):2957-68.

    Insert

    bcr/abl (P210) (ABL1, BCR Human)
    

    Use

    Retroviral

    Tags

    IRES and NGFR

    Expression

    Mammalian

    Mutation

    Tyrosine 177 mutated to Phenylalanine (Y177F)

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pHAGE-EGFR-L747_P753delinsS

    Plasmid

    #116268

    Purpose

    Lentiviral expression of EGFR L747_P753delinsS

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L747_P753delinsS

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Kir6.2 K+ channel scFv [N363/71]

    Plasmid

    #190546

    Purpose

    Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_CDK4_p.R24C

    Plasmid

    #82175

    Purpose

    Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R24C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD649-HAsp-ILDR2-COMP5AP-AviTag-9xHis

    Plasmid

    #157127

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ILDR2 (ILDR2 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIGF1_P2 Promoter-FLuc

    Plasmid

    #154267

    Purpose

    Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P2 Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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