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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJCble

Plasmid

#185861

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2S

Plasmid

#185865

Purpose

Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ265H

Plasmid

#149554

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

hAID-ziSpyMacCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266B

Plasmid

#149555

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ziSpyMacCas9(D10A)-PmCDA1-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A-SRDX

Plasmid

#129682

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b-SRDX

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ291-D573A-SRDX

Plasmid

#129683

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b-SRDX

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

D573A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK110

Plasmid

#110576

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP6 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK112

Plasmid

#110577

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP12b Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK113

Plasmid

#110578

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP32 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-U6a-U6c-sod2 guide

Plasmid

#107604

Purpose

An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod1 guide

Plasmid

#107603

Purpose

An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-uqcrc1 guide

Plasmid

#107602

Purpose

An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

uqcrc1 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4pBS3-Pleum

Plasmid

#60330

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Gal4pBS1-Pleum

Plasmid

#59993

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-B5

Plasmid

#31709

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-363 (Mir363 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S204A)

Plasmid

#17699

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Expression

Mammalian

Mutation

S204A

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only