We narrowed to 9,608 results for: FIE

1. pBSMrnaSeph

   Plasmid

   #67507

   Purpose

   Express the tRNAMet scaffold with the sephadex aptamer tag

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet with sephadex aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMIG-w

   Plasmid

   #12282

   Purpose

   a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

   Depositor

   Luk Parijs

   Article

   Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pPEE13

   Plasmid

   #128359

   Purpose

   Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-GCaMP6s

   Plasmid

   #135418

   Purpose

   Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

   Depositor

   Rylan Larsen

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter + lac operator

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p156

   Plasmid

   #186179

   Purpose

   Shotgun Metagenomic Spike-in

   Depositor

   Karsten Zengler

   Article

   Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

   Insert

   synDNA 156
   

   Use

   Synthetic Biology and Unspecified; Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_RTX_(exo-)

   Plasmid

   #102786

   Purpose

   Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase with 3'-5' deficient exonuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLxR5

   Plasmid

   #149465

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFCiR5

   Plasmid

   #149479

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSK

    Plasmid

    #67504

    Purpose

    Express the tRNALys scaffold

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pKCyR8

    Plasmid

    #149494

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymR(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-TODestRFP_RNAseH1

    Plasmid

    #65785

    Purpose

    expresses RFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    RFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd5-B1-deltaE1-GFP

    Plasmid

    #179203

    Purpose

    Block 1 with deletion in E1 and insertion of GFP expression cassette

    Depositor

    Fred Bunz

    Article

    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

    Insert

    Modified Block 1 with deletion in E1 and insertion of GFP expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pComb3HSS

    Plasmid

    #64133

    Purpose

    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet1_CylLS_CylM

    Plasmid

    #208760

    Purpose

    Expresses modified cytolysin S (mCylLS) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLS
    CylLM
    

    Use

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWLxR5

    Plasmid

    #149490

    Purpose

    Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FIRES-PBCS-CLV3

    Plasmid

    #228697

    Purpose

    Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE1 SENSOR
    

    Use

    Synthetic Biology and Unspecified

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    hpgk

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. FIREC-PBCS-MC2

    Plasmid

    #228698

    Purpose

    Control cassette FIRE1, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE1 CONTROL
    

    Use

    Synthetic Biology and Unspecified

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRXTN

    Plasmid

    #47916

    Purpose

    pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.

    Depositor

    James Iglehart

    Article

    Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. EasyFusion eGFP

    Plasmid

    #112848

    Purpose

    General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    EGFP
    

    Use

    Unspecified; General donor

    Tags

    Expression

    Mutation

    Promoter

    No

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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