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pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179060

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179052

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179051

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)

Plasmid

#178987

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRha-rsw-EYFP

Plasmid

#182928

Purpose

EYFP driven by inducible promoter Prha-rsw

Depositor

Himadri Pakrasi

Publication

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

EYFP

Promoter

Prha-rsw

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

Zhe J. Liu

Publication

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Publication

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Expression

Bacterial

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7523

Plasmid

#169090

Purpose

MoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unit

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvDODA1-Ocs terminator

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7535

Plasmid

#169089

Purpose

MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvCYP76AD1-Nos terminator

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNVLTv2

Plasmid

#169245

Purpose

Generic suicide vector for knockouts and integrations

Depositor

Brian Pfleger

Publication

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Expression

Bacterial

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-NEK9W967A

Plasmid

#168271

Purpose

Expresses LIR (LC3-interacting region) mutant NEK9

Depositor

Noboru Mizushima

Publication

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 979 to alanine

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Publication

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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