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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,354 results for: FIE

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Showing: 5661 - 5680 of 10354 results

  3. pHT-497

    Plasmid
    #87756
    Purpose
    Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)
    Depositor
    Yu-Wen Hwang
    Insert
    Gene coding for rat DYRK1A kinase domain (residues 1-497)
    Tags
    6X histidine tag
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase promoter
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87756-plasmid-map-sequence-id-173678

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  4. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

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  5. Vimentin/pBSSK+

    Plasmid
    #98133
    Purpose
    Vimentin (NM_003380) in pBSSK+
    Depositor
    Rita Shiang
    Insert
    Vimentin (VIM Human)
    Use
    Unspecified
    Available Since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98133-plasmid-map-sequence-id-189654

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  6. pET21_Dengue-NS2B-NS3_Gibbs_GTM_001

    Plasmid
    #254043
    Purpose
    Expresses Dengue Virus NS2B-NS3 protease as a fusion in E. coli.
    Depositor
    Gaetano Montelione
    Insert
    NS2B-NS3 fusion
    Tags
    10xHis-GlySer Linker-TEV
    Expression
    Bacterial
    Mutation
    GS Linker between NS2B and NS3
    Promoter
    T7
    Available Since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254043-plasmid-map-sequence-id-505757

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  7. pLCHKO_CRASP_hgERN1_XBP1

    Plasmid
    #250297
    Purpose
    Vector for producing lentivirus to generate cell lines expressing ERN1 targeting gRNA and XBP1 minigene
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Insert
    ERN1 gRNA and XBP1 minigene
    Expression
    Mammalian
    Available Since
    April 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    250297-plasmid-map-sequence-id-502164

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  8. pRDB_031

    Plasmid
    #245331
    Purpose
    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
    Depositor
    John Doench, David Root
    Insert
    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245331-plasmid-map-sequence-id-488051

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  9. pMV_AA100

    Plasmid
    #245322
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD55
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245322-plasmid-map-sequence-id-488050

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  10. pRDB_245

    Plasmid
    #245332
    Purpose
    Cas12a CRISPRko positive control guide; targets CD46
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245332-plasmid-map-sequence-id-488043

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  11. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

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  12. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  13. pRDB_247

    Plasmid
    #245334
    Purpose
    Cas12a CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245334-plasmid-map-sequence-id-488032

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  14. pMV_AA097

    Plasmid
    #245319
    Purpose
    Cas12a CRISPRko positive control; targets CD46, CD47, CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46, CD47, CD55 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245319-plasmid-map-sequence-id-488024

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  15. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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  16. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  17. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  18. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

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  19. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203977-plasmid-map-sequence-id-403301

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  20. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

    Plasmid
    #198022
    Purpose
    LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population
    Depositor
    Donald Kohn
    Insert
    CD3D c.202C>T-cDNA
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198022-plasmid-map-sequence-id-390932

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Showing: 5661 - 5680 of 10354 results