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pSB700 lenti gRNA EBFP2

Plasmid

#167905

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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C278

Plasmid

#174356

Purpose

For introducing multiple copies of OsTIR1 with the piggyBac system

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

OsTIR1

Tags

Myc

Expression

Mammalian

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE506

Plasmid

#153279

Purpose

Intermediate sgRNA array cloning vector position 4

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE505

Plasmid

#153278

Purpose

Intermediate sgRNA array cloning vector position 3

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-Ad5-SpyCatcher

Plasmid

#243751

Purpose

For affinity purification of A5 knob fused to SpyCatcher

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Ad5-SpyCatcher

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_tNGFR_ZeoR

Plasmid

#212648

Purpose

piggyBac vector to express tNGFR with zeocin resistance

Depositor

Paul Blainey

Publication

Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.

Insert

tNGFR

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3A

Plasmid

#183283

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3A

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP1

Plasmid

#183323

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP1

Use

Lentiviral

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRA

Plasmid

#183313

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRA

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH1

Plasmid

#183300

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH1

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK151

Plasmid

#183127

Purpose

BTK Type 4 reverse promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse CP25 promoter and forward rpoC terminator for dsRNA expression

Use

Synthetic Biology

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD208

Plasmid

#159762

Purpose

Expression vector for wild-type Fc-fused ACE2(740)

Depositor

Jim Wells

Publication

Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE509

Plasmid

#153281

Purpose

End-linker for cloning 3 sgRNA arrays into any recipient vector

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

linker

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE510

Plasmid

#153282

Purpose

End-linker for cloning 4 sgRNA arrays into any recipient vector

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

linker

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK300

Plasmid

#110592

Purpose

BTK Type 4 terminator for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

rpoC

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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