We narrowed to 5,917 results for: SPI

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc10-ZFD

Plasmid

#243743

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with C half of a zinc finger deaminase (ZFD) on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-ZFD

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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297_pETcon_SARS2_Omicron-BA286

Plasmid

#222231

Purpose

yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 BA.2.86 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

Plasmid

#186274

Purpose

Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas6-RT-Cas1

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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as-mOPN-PB0

Plasmid

#106450

Purpose

Bacterial expression of antisense to murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

antisense-osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

antisense

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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TAL3527

Plasmid

#47246

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive)-right (rp2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3526

Plasmid

#47245

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive)-left (rp2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Article

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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WN10151

Plasmid

#80441

Purpose

Expresses human codon-optimized dead (832A) LbCpf1

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Insert

dead (D832A) LbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-Flag-WIP

Plasmid

#11772

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP (WIPF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 S

Plasmid

#158371

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 S (S )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-myc-ASPM MTB

Plasmid

#27668

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Tags

GFP

Expression

Mammalian

Mutation

Contains amino‑terminal putative microtubule bind…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pFUGW-RapACR-EYFP

Plasmid

#111075

Purpose

Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR

Use

Lentiviral

Tags

EYFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-RapACR_T111C-EYFP

Plasmid

#119195

Purpose

Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR fast mutant T111C

Use

Lentiviral

Tags

EYFP

Mutation

changed threonine 111 to cysteine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSFG-TFII-I-GFP beta

Plasmid

#22200

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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