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We narrowed to 9,991 results for: FIE

Showing: 8551 - 8580 of 9991 results
  1. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. CFP(1-158)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55619
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    CFP-(1-158)-gamma-10 (GNG10 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. CFP(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55617
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    CFP(1-158)-gamma-5 (GNG5 Aequorea victoria, Bovine)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. CFP(1-158)-gamma-7 in pcDNAI/Amp

    Plasmid
    #55618
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    CFP(1-158)-gamma-7 (GNG7 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. CFP(1-158)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55620
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    CFP(1-158)-gamma-11 (GNG11 Aequorea victoria, Bovine)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. CFP(1-158)-gamma-12 in pcDNAI/Amp

    Plasmid
    #55621
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    CFP(1-158)-gamma-12 (GNG12 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-12 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. Cer(1-158)-gamma-1 in pcDNAI/Amp

    Plasmid
    #55623
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. Cer(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55624
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. Cer(1-158)-gamma-7 in pcDNAI/Amp

    Plasmid
    #55626
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-7 (GNG7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. Cer(1-158)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55627
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-10 (GNG10 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. Cer(1-158)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55628
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-11 (GNG11 Aequorea victoria, Bovine)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. Cer(1-158)-gamma-12 in pcDNAI/Amp

    Plasmid
    #55629
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-12 (GNG12 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-12 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. YFP(159-238)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55191
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-5. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(159-238)-gamma-5 (GNG5 Aequorea victoria, Bovine)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. YFP(159-238)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55193
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(159-238)-gamma-11 (GNG11 Aequorea victoria, Bovine)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  17. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available Since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  19. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  20. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  21. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  22. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  23. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  24. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  25. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  26. MB 93C1 thsS(t3)R-Bxb1_P7-bARGSer

    Plasmid
    #232469
    Purpose
    Optimized thiosulfate sensor with recombinase switch and acoustic reporter genes (bARGSer)
    Depositor
    Inserts
    thsS(t3)
    thsR
    PphsA342
    bARGSer
    AxeTxe
    Bxb1 integrase
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23104 (ttgacagctagctcagtcctaggtattgtgctagc), J23…
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8551 - 8580 of 9991 results