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pIVT-evoBxb1

Plasmid

#232747

Purpose

IVT template for making modRNA encoding evoBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1-I87L

Plasmid

#232749

Purpose

IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

I87L

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP yellow RNA Lantern

Plasmid

#226096

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_TRE-mScarlet

Plasmid

#229796

Purpose

Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mScarlet

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_TRE-jRCaMP1b

Plasmid

#229798

Purpose

Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

jRCaMP1b

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Expression

Bacterial

Promoter

Tetracycline resistance cassette promoter (consti…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG2A HRD

Plasmid

#207540

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG2A locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP red RNA Lantern

Plasmid

#226097

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-FRB

Plasmid

#194455

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FRB (MTOR Human)

Tags

GAG

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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K560-Halo-Strep

Plasmid

#219681

Purpose

Mammalian expression of KIF5B(1-560)-HaloTag-Strep

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Tags

HaloTag, Strep tag

Expression

Mammalian

Mutation

1-560

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-573)

Plasmid

#219683

Purpose

Mammalian expression of CLIPf-KIF5A(1-573)

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf

Expression

Mammalian

Mutation

1-573

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A

Plasmid

#200790

Purpose

Mammalian expression of CLIPf-KIF5A

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

delta UIM

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GST-beta catenin tag

Plasmid

#186422

Purpose

Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

GST-beta catenin tag

Tags

6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSN668

Plasmid

#219962

Purpose

Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

Depositor

Shinsuke Niwa

Insert

codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)

Tags

sfGFP::2xStrepII tag

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-HsTtyh1-TwinStrep-His

Plasmid

#211332

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Tweety homology protein 1

Use

Lentiviral

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

W417R

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAPtag5-IgK-NCAN ELS

Plasmid

#219813

Purpose

Expresses His-tagged NCAN-ELS to produce recombinant protein

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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