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pcDNA3 Ub2-UBB+1

Plasmid

#23968

Depositor

Nico Dantuma

Publication

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Publication

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-Annexin-12

Plasmid

#53991

Purpose

Localization: Plasma Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Annexin (ANXA4 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
xfz8/pCS2+

Plasmid

#16321

Depositor

Peter Klein

Publication

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-8 (fzd8.S Frog)

Use

Vertebrate expression

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

Plasmid

#176932

Purpose

Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108) (GMNN, SLBP Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Publication

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-SCYL1

Plasmid

#23614

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL1 (SCYL1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

Plasmid

#187957

Purpose

FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Publication

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP12_F36V-KRAB-SpdCas9-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS

Expression

Mammalian

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisA-H248Y-Sirt3

Plasmid

#24917

Depositor

Toren Finkel

Publication

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

His248 mutated to Tyr(Y)

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB

Plasmid

#14150

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FLAG-GRP78-DeltaER

Plasmid

#238416

Purpose

Expresses GRP78 lacking the ER signal peptide in mammalian cells

Depositor

Amy Shiu Lee

Publication

Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The first 18 amino acids which is the ER signal p…

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Publication

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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