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pMCh-1547

Plasmid

#82434

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO1

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA7_no stop

Plasmid

#83872

Purpose

Gateway donor vector for IAA7 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA7 (IAA7 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Expression

Bacterial

Promoter

Ptb38

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1/V5-His TOPO TA mouse Mns1

Plasmid

#42899

Depositor

Jeremy Wang

Article

Zhou et al PLoS Genet. 2012;8(3):e1002516. doi: 10.

Insert

Mns1 (Mns1 Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
B. subtilis Single Gene Deletion Library--Kanamycin

Plasmid Kit

#1000000115

Purpose

B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.

Depositor

Carol Gross

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only
pGMCtN-TSC10M1-sspB

Plasmid

#49521

Purpose

Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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His-FGE

Plasmid

#75147

Purpose

Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

Depositor

Richard Fishel

Article

Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

Insert

FGE

Tags

Histidine tag and Thrombin

Expression

Bacterial

Mutation

N/A

Promoter

T7

Available Since

July 8, 2016

Availability

Academic Institutions and Nonprofits only
pKan-AID*-9myc

Plasmid

#99522

Purpose

C-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-GFP

Plasmid

#99521

Purpose

C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

GFP

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6HA

Plasmid

#99520

Purpose

C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xHA

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE

Plasmid

#112872

Purpose

binary vector for ABE expression in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA cassette

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6FLAG

Plasmid

#99519

Purpose

C-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xFLAG

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry-UBQ

Plasmid

#78932

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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peCALWY-4

Plasmid

#22236

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

C418S mutation linker consists of 9 GGSGGS repea…

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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