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pCEFL-EGFP-TEADiv2

Plasmid

#228872

Purpose

Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Tags

GFP

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-TIS11B CPM2

Plasmid

#121143

Purpose

Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pShGSK3bE

Plasmid

#37526

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

GSK3b (GSK3B Human)

Tags

HA

Expression

Mammalian

Promoter

SRa promoter

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Src

Plasmid

#19965

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

Src (SRC Chicken)

Expression

Mammalian

Mutation

K295M

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA-iRPT

Plasmid

#140763

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassette

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPS1358

Plasmid

#8918

Depositor

Pamela Silver

Article

Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

Insert

HRP1 (HRP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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KHBD00319

Plasmid

#34444

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2102 (cas Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pBS chick LH-2B [TJ#34]

Plasmid

#16271

Depositor

Thomas Jessell

Article

Liem et al Cell. 1997 Oct 3. 91(1):127-38.

Insert

LH2B (LHX9 Chicken)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pMDW108

Plasmid

#227194

Purpose

Template for multi-nucleosome array

Depositor

Michelle Wang

Article

Le et al Cell. 2019 Oct 17;179(3):619-631.e15. do

Insert

64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCEFL-HAx2-TEADiv2

Plasmid

#228873

Purpose

Expression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Tags

2xHA

Expression

Mammalian

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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PaCasp3

Plasmid

#166052

Purpose

a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Porites astreiodes caspase-3

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGJM015

Plasmid

#128549

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 5

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM013

Plasmid

#128548

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 4

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM010

Plasmid

#128546

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 2

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM09

Plasmid

#128545

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 1

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Synthetic, Mustard Weed, Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR161Q

Plasmid

#92413

Purpose

hQPRTase harboring R161Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161Q

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIAhqprtR161G

Plasmid

#92412

Purpose

hQPRTase harbouring R161G mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161G

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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