We narrowed to 2,693 results for: CCS

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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gCH128 (non-targeting crRNA)

Plasmid

#217339

Purpose

Non-targeting crRNA

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crNT-3

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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EK0450 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Plasmid

#233374

Purpose

LIDAR reporter RNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM101 CMV-TO-ADAR2dd(E488Q,T501A)-MCP

Plasmid

#233432

Purpose

ADAR2dd(E488Q,T501A)-MCP (positive control that fuses ADAR2dd and MCP on the same protein)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-tdMCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

Plasmid

#213117

Purpose

Expression of a faster-running MinE mutant in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE(L4E)-EGFP (minE Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Mutation

L4E

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

Plasmid

#213116

Purpose

Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

FRB-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIS1-Eef25UTR-renilla

Plasmid

#38235

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2 5'UTR / Renilla (Eef2 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Eef2 5' UTR fused to renilla ORF

Promoter

Endogenous Eef2

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

Plasmid

#213119

Purpose

Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

Plasmid

#213118

Purpose

Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

PKAsub-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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EK0396 BFPmut-stop-t70587 (pEAQ)

Plasmid

#191171

Purpose

EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587 (plant)

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBS hTSP-5

Plasmid

#13002

Depositor

Jack Lawler

Article

Newton et al Genomics. 1994 Dec . 24(3):435-9.

Insert

COMP (COMP Human)

Expression

Bacterial

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and UBC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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MX1_pCSdest

Plasmid

#53951

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MX1 (MX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

Plasmid

#191161

Purpose

Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP.t2

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe neo TGFBR3-HA

Plasmid

#83095

Purpose

Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

TGFBR3 (TGFBR3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)

Plasmid

#191138

Purpose

Expression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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