We narrowed to 1,368 results for: GPCR;

1. pFB-CG-LYCHOS WT-HA-GFP

   Plasmid

   #199687

   Purpose

   Expression of LYCHOS WT in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Tags

   10xHis, EGFP, HA, HRV 3C, and His

   Expression

   Insect

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-LYCHOS (GPR155) ∆DEP-HA

   Plasmid

   #199683

   Purpose

   Transient expression of LYCHOS (GPR155) ∆DEP-HA

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   DEP domain deleted

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 LYCHOS (GPR155) Y551A-HA

   Plasmid

   #199676

   Purpose

   Transient expression of LYCHOS (GPR155) Y551A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y551 mutated to A

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a LZ LED WT-FLAG

   Plasmid

   #199679

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED WT (GPR155 Human)
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-LYCHOS (GPR155) P44A-HA

   Plasmid

   #199675

   Purpose

   Transient expression of LYCHOS (GPR155) P44A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P44 mutated to A

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-LYCHOS (GPR155) E48Q-HA

   Plasmid

   #199662

   Purpose

   Transient expression of LYCHOS (GPR155) E48Q mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E48 mutated to Q

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5-LYCHOS (GPR155) F43I-HA

   Plasmid

   #199674

   Purpose

   Transient expression of LYCHOS (GPR155) F43I mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F43 mutated to I

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRUPATH Triple GaoB

   Plasmid

   #196052

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoB-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB-CG-LYCHOS FP>IA-HA-GFP

    Plasmid

    #199688

    Purpose

    Expression of LYCHOS FP>IA mutant in insect cells

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Tags

    10xHis, 6xHis, EGFP, HA, and HRV 3C

    Expression

    Insect

    Mutation

    F43 and P44 mutated to I and A respectively

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a LZ LED 4CA-FLAG

    Plasmid

    #199681

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED 4CA (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    C595.604.629.638 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a LZ LED Y551A-FLAG

    Plasmid

    #199680

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED Y551A (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Y551 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRUPATH Triple Gai3

    Plasmid

    #196050

    Purpose

    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRUPATH Triple Gagust

    Plasmid

    #196054

    Purpose

    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-MRE/CRE/SRE

    Plasmid

    #236751

    Purpose

    Use as a MRE/CRE/SRE reporter for luciferase assays to measure GPCR activation.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    MRE/CRE/SRE
    

    Use

    Luciferase

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge