We narrowed to 42,551 results for: Ina

1. SpyCatcher002-oHept

   Plasmid

   #124671

   Purpose

   Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHept
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-Talin head-SpyCatcher003

   Plasmid

   #133566

   Purpose

   Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   EGFP-Talin head-SpyCatcher003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpyDock

   Plasmid

   #124618

   Purpose

   Expresses SpyDock to bind SpyTag non-covalently for affinity purification

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyDock
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpyTag003-Talin rod-mCherry

   Plasmid

   #133567

   Purpose

   Expresses SpyTag003-Talin rod-mCherry in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-Talin rod-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Use

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SpyTag002 DA-MBP

   Plasmid

   #102832

   Purpose

   Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002 DA-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Reactive aspartic acid has been mutated to an ala…

   Promoter

   T7

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpySwitch

    Plasmid

    #184225

    Purpose

    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpySwitch
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Sctrans-PP

    Pooled Library

    #153101

    Purpose

    CRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Sctrans-PP-Grp1

    Pooled Library

    #153102

    Purpose

    CRISPR library targets a sub-pool of transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

14. Sctrans-PP-Grp2

    Pooled Library

    #153103

    Purpose

    CRISPR library targets a sub-pool of transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

15. Sctrans-PP-Grp3

    Pooled Library

    #153104

    Purpose

    CRISPR library targets a sub-pool of transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

16. Sctrans-PP-Grp4

    Pooled Library

    #153105

    Purpose

    CRISPR library targets a sub-pool of transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

17. Sctrans-PP-Grp5

    Pooled Library

    #153106

    Purpose

    CRISPR library targets a sub-pool of transporter genes in S. cerevisiae

    Depositor

    Irina Borodina

    Article

    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

18. Mouse metabolism knockout library

    Pooled Library

    #160129

    Purpose

    CRISPR library targets mouse metabolic genes

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

19. Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

    Pooled Library

    #171531

    Purpose

    Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB)

    Depositor

    Chunliang Li

    Article

    Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

20. Human Epigenetic Knockout Library

    Pooled Library

    #162256

    Purpose

    Lentiviral CRISPR library targets human epigenetic genes

    Depositor

    Kivanc Birsoy

    Article

    Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only