Addgene

We narrowed to 1,058 results for: agl

Showing: 561 - 580 of 1058 results

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX 4T-1 GST-Ago2 S387A

Plasmid

#60656

Purpose

Expresses GST-Ago2 S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

S387A

Promoter

tac

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xE

Plasmid

#92008

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1

Plasmid

#133978

Purpose

mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-RNF168 delta MIU1/MIU2

Plasmid

#133981

Purpose

mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LRG/Rosa26

Plasmid

#105510

Purpose

Lentiviral expression of Rosa26 targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387E

Plasmid

#60655

Purpose

GFP-Ago2 S387E mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387E

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPB CAG Gfi1:FLAG-IRES-EGFP

Plasmid

#153945

Purpose

piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Gfi1 (Gfi1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU2

Plasmid

#133979

Purpose

mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU2

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPB CAG SMARCA4 T910M-IRES-EGFP

Plasmid

#153949

Purpose

piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T910M

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xA

Plasmid

#92010

Purpose

Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1622 NOLC1 sgRNA 1

Plasmid

#234833

Purpose

sgRNA 1 for NOLC1 STITCHR insertion

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360L

Plasmid

#233619

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Leu (DDX3X Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Gln360Leu

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360P

Plasmid

#233620

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Pro (DDX3X Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Gln360Pro

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E

Plasmid

#91999

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S824E-S831E

Plasmid

#92000

Purpose

Expresses FLAG-HA-AGO2 (S824E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S828E-S831E

Plasmid

#92001

Purpose

Expresses FLAG-HA-AGO2 (S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge