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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,583 results for: construct

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Showing: 561 - 580 of 8583 results

  1. rox Neo* rox

    Plasmid
    #134322
    Purpose
    rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Dre/rox
    Expression
    Bacterial and Mammalian
    Available Since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    134322-plasmid-map-sequence-id-316179

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  2. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  3. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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  4. pTP63-donor

    Plasmid
    #132477
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132477-plasmid-map-sequence-id-273771

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  5. pE4F1.1.0-gDNA

    Plasmid
    #132451
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    E4F1 (E4F1 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132451-plasmid-map-sequence-id-266819

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  6. pJK139

    Plasmid
    #73315
    Purpose
    Pas2p peroxisomal membrane biogenesis protein
    Depositor
    T. J. Kappock
    Insert
    Pas2p peroxisomal membrane targeting protein
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    Encodes a glycine-204 to serine mutation
    Promoter
    AOX1
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73315-plasmid-map-sequence-id-140431

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  7. pJK137

    Plasmid
    #73313
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    5'-AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73313-plasmid-map-sequence-id-135010

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  8. pJK538

    Plasmid
    #72445
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72445-plasmid-map-sequence-id-133868

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  9. mApple-Desmoglein2-C-18

    Plasmid
    #54889
    Purpose
    Localization: Desmosomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Desmoglein2 (DSG2 Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. mApple-Desmocollin1-N-18

    Plasmid
    #54886
    Purpose
    Localization: Cadherin, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Desmocollin1 (DSC1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44095-plasmid-map-sequence-id-61672

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  16. mCherry-H3-23

    Plasmid
    #55058
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    H3 (H3C2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55058-plasmid-map-sequence-id-424732

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  17. mCherry-Centrin2-N-10

    Plasmid
    #55018
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55018-plasmid-map-sequence-id-371039

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  19. mCherry-Beta-Catenin-20

    Plasmid
    #55001
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55001-plasmid-map-sequence-id-300582

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  20. mCherry-Rab11a-7

    Plasmid
    #55124
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab11a (RAB11A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55124-plasmid-map-sequence-id-127231

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Showing: 561 - 580 of 8583 results