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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA3

Plasmid

#107075

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA2

Plasmid

#107074

Purpose

M. sedula PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA1

Plasmid

#107073

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-SCN4A-FLAG

Plasmid

#244365

Purpose

3' AAVLINK plasmid for SCN4A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

SCN4A (SCN4A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG

Plasmid

#244297

Purpose

3' AAVLINK plasmid for CACNA1C expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CACNA1C (CACNA1C Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR153

Plasmid

#226812

Purpose

Tet-inducible lentiviral vector for 2xHA GPR153 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP S624A

Plasmid

#207402

Purpose

Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Serine 624 to Alanine. It's a protea…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP A657S

Plasmid

#207403

Purpose

Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Alanine 657 to Serine. It's an endop…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl2-pEGFP-C1

Plasmid

#177416

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGREAT17

Plasmid

#170905

Purpose

GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

p35SoTMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_N82A

Plasmid

#156183

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1-RAMP4

Plasmid

#139863

Purpose

Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1-RAMP4 (UFL1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of UFL1 (full length) with RAM…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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CLV1 L2_pECIA14

Plasmid

#114900

Purpose

Prey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G75820 (CLV1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK1 A6_pECIA14

Plasmid

#114767

Purpose

Prey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G71830 (SERK1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BRI1 BRI1_pECIA2

Plasmid

#115086

Purpose

Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39400 (BRI1 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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