We narrowed to 12,024 results for: pcdna

1. pcDNA3.1 Flag YFP hNKCC1 (NT17)

   Plasmid

   #49085

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available Since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1013 pcDNA3 T7 Akt1 T308A S473A

   Plasmid

   #9030

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T308A S473A

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Flag-Siah2 Ring Mutant

   Plasmid

   #122042

   Purpose

   express Siah2 RM

   Depositor

   Ze'ev Ronai

   Insert

   Siah2 RM (Siah2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ring mutant deletion H98, L99A, V100G, C101A

   Promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 CMV 3xFG eIF4G1 +MIC

   Plasmid

   #158776

   Purpose

   pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (with microexon)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Au1-mTOR-Wild type

   Plasmid

   #26036

   Depositor

   Fuyuhiko Tamanoi

   Article

   Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

   Insert

   mammalian target of rapamycin-wild type (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1 His-NLRP1 codon-optimized

   Plasmid

   #166833

   Purpose

   Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His

   Plasmid

   #83452

   Purpose

   Full-length murine PC1 for Eukaryotic Expression (982)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   polycystin-1 (Pkd1 Mouse)
   

   Use

   Tags

   2xMyc, 3xFlag, and 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-NES-DEVD-mCardinal-NLS

   Plasmid

   #164052

   Purpose

   Fluorescent reporter for caspase-3

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-mCardinal-NLS
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

    Plasmid

    #183249

    Purpose

    Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    Anti-Her2 scFvFc (FRP5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-mouse PKA-RIIalpha-mEGFP

    Plasmid

    #45527

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII alpha tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 CMV sTurboID (C)-Arrestin-GFP

    Plasmid

    #153010

    Purpose

    Expresses split-TurboID C-terminal fragment fused to arrestin

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C)(G74)-Arrestin
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

    Plasmid

    #171436

    Purpose

    Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyn-dInPAkt(R23/25A)

    Plasmid

    #181835

    Purpose

    Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Arginines 23 and 25 in PH domain mutated to alani…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

    Plasmid

    #166794

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE248 pCDNA3.1+zeo-VSV-KLHL12

    Plasmid

    #16761

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mouse PKA-Calpha-mEGFP

    Plasmid

    #45521

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA catalytic subunit alpha tagged by mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCDNA3 Flag p38 alpha (agf)

    Plasmid

    #20352

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 180 and Tyr 182 with Ala and Phe resp…

    Promoter

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG- gate-pGK-HYG

    Plasmid

    #107397

    Purpose

    Gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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