We narrowed to 8,588 results for: Dos
-
Plasmid#200160PurposePrig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFPDepositorAvailable sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
TagsmNeptuneExpressionWormPromoterPtwk-40Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH3830
Plasmid#200038PurposePrig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFPDepositorAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXY349
Plasmid#200262PurposeExpressing FtsW-TagRFP-T in bacterial cellsDepositorAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
OR1-consensus
Plasmid#208059PurposeConsensus OR1 engineered from human OR1 family membersDepositorInsertOR1-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR2-consensus
Plasmid#208060PurposeConsensus OR2 engineered from human OR2 family membersDepositorInsertOR2-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR4-consensus
Plasmid#208061PurposeConsensus OR4 engineered from human OR4 family membersDepositorInsertOR4-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FUS-FusionRed
Plasmid#188393PurposeCu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cellsDepositorAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1ΔR198A/R210A-HA-Puro
Plasmid#193769PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1ΔR198A/R210A (CDT1 Human)
UseLentiviralTagsHAMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-mCherry-Puro
Plasmid#193766PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-HA-Puro
Plasmid#193767PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-HA (CDT1 Human)
UseLentiviralTagsHAMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1Δ499-546-HA-Puro
Plasmid#193768PurposeDoxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1Δ499-546 (CDT1 Human)
UseLentiviralTagsHAMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
TagsAcGFP1ExpressionInsectPromoterAc5Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
noSIIN
Plasmid#185666PurposeshApc-expressing lentiviruses with no expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H150
Plasmid#170345Purpose6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H94
Plasmid#170341PurposeGFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)
Plasmid#133792PurposeU6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015DepositorInsertSt3Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Muller et al. Molecular T…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCPP6533
Plasmid#128734PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal Cya tagDepositorTypeEmpty backboneExpressionBacterialAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only