We narrowed to 40,006 results for: lat

1. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121184

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   CMV-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ExRai-AMPKAR(T/A)

   Plasmid

   #192447

   Purpose

   Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR(T/A)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Thr 6 in AMPK substrate mutated to Ala.

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. V5-LOV-Turbo-ERM

   Plasmid

   #199664

   Purpose

   expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   SEC61B and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMALp2x-CARP-1

   Plasmid

   #41727

   Depositor

   Koji Okamoto

   Article

   Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   clam ADP-ribosylating protein-1
   

   Use

   Bacterial subcloning

   Tags

   Expression

   Mutation

   deleted 5'-UTR nucleotides 1-64

   Promoter

   pMB1

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS_cAMPFIRE-M

   Plasmid

   #182284

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEG302 22aa SunTag VP64 nog

   Plasmid

   #120251

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-C1_SEPT9 i1

   Plasmid

   #71622

   Purpose

   mammalian expression of human SEPT9 with an mCherry fusion

   Depositor

   Elias Spiliotis

   Article

   Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGal4x5-E4T-G210

   Plasmid

   #171077

   Purpose

   The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4-E4-Gless-cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVT-p53DD

    Plasmid

    #232751

    Purpose

    IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    p53DD
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    deletion of DNA binding domain

    Promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSV human p85 alpha (HA tag)

    Plasmid

    #11499

    Depositor

    Ronald Kahn

    Article

    Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

    Insert

    p85 alpha (PIK3R1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEG302 22aa SunTag NtDRMcd nog

    Plasmid

    #115488

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Leo1

    Plasmid

    #11061

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    Leo1 (LEO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS_cAMPFIRE-H

    Plasmid

    #182285

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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