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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Article

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mouse, Mustard Weed)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Purpose

Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pTR-UF11 (AAV.A10.T)

Viral Prep

#157970-AAV.A10.T

Purpose

Ready-to-use AAV6(dbY-F+T-V) in TMN200 particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter

Depositor

Sergei Zolotukhin

Article

Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

Promoter

chimeric CMV/Chicken Beta actin (CBA)

Tags

GFP

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only
pTW084b

Plasmid

#116894

Purpose

Activity-independent SE-PACE AP

Depositor

David Liu

Article

Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

Inserts

g3N
434-cI/scFv m3

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK003

Plasmid

#65110

Purpose

Encodes ConL1 as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConL1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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p5xNF-kb_RE

Plasmid

#118050

Purpose

GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

5 copies of the NF-kb DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162228

Purpose

Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG16LaG2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Reporter-2

Plasmid

#180219

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTW051d

Plasmid

#116892

Purpose

low stringency SE-PACE AP

Depositor

David Liu

Article

Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

Inserts

g3c
T7 RNAP C-term R632S/Q649S

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG291

Plasmid

#115605

Purpose

surface-displayed Null, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG288

Plasmid

#115607

Purpose

surface-displayed Ag3, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG287

Plasmid

#115606

Purpose

surface-displayed Ag2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPROBE-gfp[AAV]

Plasmid

#40168

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPC1000

Plasmid

#174829

Purpose

Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Inserts

CRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

EF1a and mouse U6

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D-5

Plasmid

#107866

Purpose

Bacterial expression of PIS, PI4K and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD and proD (in operon)

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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