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Anti-Cavbeta2 Ca2+ channel [N8B/1R]

Plasmid

#149458

Purpose

Mammalian Expression Plasmid of anti-Cavbeta2 Ca2+ channel (Rat). Derived from hybridoma N8B/1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

Mammalian Expression of anti-Cavbeta2 Ca2+ channel (Rat) recombinant mouse monoclonal antibody (Cacnb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#190486

Purpose

Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCB268

Plasmid

#53363

Purpose

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

Depositor

Alexander Varshavsky

Article

Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

Inserts

DHFR (Dhfr Mouse)
Ub
Aβ42 (APP Human)

Tags

13xMyc, Flag, and HA

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pFB2A

Plasmid

#195283

Purpose

Bicistronic DTR and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Hygro (HBEGF Human, Synthetic)

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Tol2-PA2-CMV-AB-mCh

Plasmid

#160435

Purpose

Expresses human Amyloid Beta-mCherry in Zebrafish

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Human Amyloid Beta peptide (1-42) (APP Zebrafish, Human)

Use

Zebrafish expression

Tags

mCherry

Promoter

CMV

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

Plasmid

#231973

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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IP3 receptor, type 1 scFv [L24/21]

Plasmid

#206790

Purpose

Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/21 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-ME.2/ApoEpromoter

Plasmid

#51436

Purpose

This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

Insert

APOE apolipoprotein E (APOE, ME2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The ME.2 enhancer is fused upstream of the ApoE g…

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-KDR

Plasmid

#116754

Purpose

Lentiviral expression of KDR

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KDR (KDR Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Bcr/Abl P210-pLEF

Plasmid

#38158

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P210 (ABL1, BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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mBFP-APP-mGFP

Plasmid

#196694

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-EGR1

Plasmid

#192913

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

EGR1 (EGR1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2158

Plasmid

#141949

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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Bcr/Abl P190-pLEF

Plasmid

#38159

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Tags

GST

Expression

Mammalian

Mutation

T117M in c-ABL1

Promoter

SV40

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-E746_T751delinsA

Plasmid

#116246

Purpose

Lentiviral expression of EGFR E746_T751delinsA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

E746_T751delinsA

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel (External) [K39/25R]

Plasmid

#128620

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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