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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pHWBF01

Plasmid

#131022

Purpose

Synthetic source clone for maize Histon H2B.5 with C-terminal mCherry fusion

Depositor

Hank Bass

Article

Howe et al DNA Cell Biol. 2012 Jun;31(6):925-38. do

Insert

Histone H2B.5

Use

Cloning vector

Tags

mCherry

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Cck

Plasmid

#105989

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

cholecystokinin (Cck Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pKIOct4-Halo-IRES-Bsd

Plasmid

#115689

Purpose

Knock-in of a HaloTag fusion protein into the endogenous OCT4 (Pou5f1) protein

Depositor

David Suter

Article

Strebinger et al Mol Syst Biol. 2019 Sep;15(9):e9002. doi

Insert

OCT4-Halo-IRES-Bsd

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLBT0190

Plasmid

#172815

Purpose

Level 1 module containing OEP7-mTRQ_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-OEP7-mTRQ-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0100

Plasmid

#172789

Purpose

Level 0 module containing CT_Stop

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with stop codons.

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0150

Plasmid

#172795

Purpose

Level 0 module containing NT2_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0050

Plasmid

#172781

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0051

Plasmid

#172782

Purpose

Level 0 module containing CT_3XFLAGnYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0526-mCherryBb

Plasmid

#118241

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0526 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBbA5k-EPL95

Plasmid

#45434

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

AcrB/AcrD/AcrFa, ABO_0964

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pYC44

Plasmid

#63903

Purpose

Initial Integrative Vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FRT::3'UTRcta::NAT::FRT

Expression

Yeast

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb4EIG

Plasmid

#184683

Purpose

NIR-Fb to E.coli dihydrofolate reductase

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb4EIG

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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mEos4b-LaminA-C-18

Plasmid

#57511

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

LaminA (LMNA Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBRN168

Plasmid

#79668

Purpose

DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (1–168)/RN168

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Expression

Mammalian

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Profilin-10

Plasmid

#55440

Purpose

Localization: Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Profilin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBGN155

Plasmid

#74992

Purpose

GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

GFP (1–155)/GN155

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-Paxillin-22

Plasmid

#57970

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only