We narrowed to 11,142 results for: LIC

Showing: 5821 - 5840 of 11142 results

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI4-3×FLAG

Plasmid

#215512

Purpose

Expresses FLAG tagged human WIPI4.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUltra-MDH1-ME1

Plasmid

#184465

Purpose

Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydrogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Lentiviral

Tags

fused to T2A

Expression

Mammalian

Mutation

deletion of stop codon

Promoter

UbC

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

Plasmid

#187275

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

deltaMyosin binding domain

Promoter

U6, CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

Plasmid

#167313

Purpose

Template pDNA to prepare DD-CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

DD-CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

Plasmid

#166413

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

Protection Of Telomeres 1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

ΔOB deleted 125 a.a

Promoter

ef1-a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

Plasmid

#69052

Purpose

lentiviral expression of IKZF2 variant 2 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

splice variant 2

Promoter

UBC

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pCfB2990

Plasmid

#63645

Purpose

pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUASTattB-MKII::nlsLexADBDo

Plasmid

#64718

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKII::nlsLexADBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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RISeGFP (CC#353)

Plasmid

#16131

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Insert

eGFP

Tags

eGFP

Expression

Mammalian

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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POT4-RFP

Plasmid

#65320

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

Plasmid

#175259

Purpose

Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lox-rox-STOP-rox-lyn-mScarlet-pA-lox

Use

Cre/Lox; Dre/rox

Tags

mScarlet

Expression

Mammalian

Mutation

None (wt)

Promoter

pCAG

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGS-FCER1G-1

Plasmid

#109192

Purpose

Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Human full-length FCER1G, codon-optimized (FCER1G Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCYC1pr-GNSI (LEU)

Plasmid

#111450

Purpose

GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

Depositor

Alexey Merz

Article

Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

Inserts

CYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator

Tags

mGFP5

Expression

Yeast

Mutation

M109I (Naturally occurring SNP) and The first 21 …

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2795

Plasmid

#63650

Purpose

pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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