We narrowed to 168,468 results for: addgene

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pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.112

Plasmid

#184981

Purpose

Express -Eco1 FAA1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12

Expression

Yeast

Mutation

FAA1 donor P233stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.002

Plasmid

#184972

Purpose

Express -Eco1 ADE2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12

Expression

Yeast

Mutation

ADE2 donor P272stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.110

Plasmid

#184979

Purpose

Express -Eco1 TRP2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12

Expression

Yeast

Mutation

TRP2 donor E64stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184692

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184693

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184694

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184701

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184702

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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XLone-BSD NFIL3

Plasmid

#179512

Purpose

Tunable and temporal expression control of NFIL3

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

NFIL3 (NFIL3 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS Promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-KDEL

Plasmid

#177715

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

KDEL and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pStr-KDEL_APV-SBP-Dendra2-ER-5

Plasmid

#170679

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

APV-SBP-Dendra2-ER-5
streptavidin-KDEL

Expression

Mammalian

Promoter

IRES and T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170812

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

pCMV and T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMB204: UbC-GFP-Tg_SAP30L-WPRE

Plasmid

#168109

Purpose

Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

SAP30L (SAP30L T. guttata)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

UbC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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THI ONLY pALD4 T-

Plasmid

#161497

Purpose

Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJK528

Plasmid

#155388

Purpose

dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJK434

Plasmid

#155378

Purpose

PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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