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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 5821 - 5840 of 10083 results

  1. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. SETD7

    Plasmid
    #40746
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7 (SETD7 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    Nov. 14, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    40746-plasmid-map-sequence-id-364373

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  5. SETD2

    Plasmid
    #40741
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD2 (SETD2 Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. dABE

    Plasmid
    #124447
    Purpose
    Expresses dead Cas9 (dCas9)-ABE7.10
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dABE7.10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124447-plasmid-map-sequence-id-241059

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  9. UBC32

    Plasmid
    #25213
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc32.001.158 (UBE2K Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    25213-plasmid-map-sequence-id-461309

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  15. dCBE4-gam

    Plasmid
    #124449
    Purpose
    Expresses dead Cas9 (dCas9)-CBE4-gam
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCBE4-gam
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124449-plasmid-map-sequence-id-241051

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  16. pKK-mRuby3-TEV

    Plasmid
    #105780
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mRuby3-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105780-plasmid-map-sequence-id-203369

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  17. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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  19. mOtop1_pcDNA3

    Plasmid
    #114677
    Purpose
    This construct was used to express mouse Otop1 in HEK-293 cells
    Depositor
    Emily Liman
    Publication
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Expression
    Mammalian
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    CMV
    Available since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114677-plasmid-map-sequence-id-221605

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  20. Erk2

    Plasmid
    #40777
    Purpose
    Expression of rat Erk2 in mammalian cells
    Depositor
    Dustin Maly
    Publication
    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Erk2 (Mapk1 Rattus norvegicus)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 5821 - 5840 of 10083 results