We narrowed to 15,991 results for: LEA

1. pFSW Doc2alpha WT

   Plasmid

   #128812

   Purpose

   To make virus expressing Doc2alpha WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW Doc2beta WT

   Plasmid

   #128814

   Purpose

   To make virus expressing Doc2beta WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBK.013

   Plasmid

   #187213

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTox-EGFPsite1_NGG-PAM (BPK740)

   Plasmid

   #181746

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
   

   Use

   Selection plasmid

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXB2 (human) HIS-tag pET

   Plasmid

   #8522

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB2 (HOXB2 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Starts at AA #2; makes protein by Western

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. Myc-Cdh1

   Plasmid

   #28127

   Depositor

   Pumin Zhang

   Article

   Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

   Insert

   fzr1 (FZR1 Human)
   

   Use

   Mouse Targeting and Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pRSFDuet-1_VNp-StefinA

   Plasmid

   #182403

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCALPS-Flag-Mili-EGFP

   Plasmid

   #212576

   Purpose

   Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   Mili (Piwil2 Mouse)
   

   Use

   Lentiviral

   Tags

   5x Flag and SNAP tag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV V5-Tmem214

   Plasmid

   #175153

   Purpose

   Lentiviral expression of V5-tagged mouse Tmem214

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem214 (Tmem214 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-Oct4

    Plasmid

    #136611

    Purpose

    Dox-inducible lentiviral vector expressing mouse Oct4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-mCh-ACTB-P2A-EGFP

    Plasmid

    #165158

    Purpose

    Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    mCherry-ACTB-P2A-EGFP (ACTB Human)
    

    Use

    Retroviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hy_pMT Flag-Hel25E WT

    Plasmid

    #110123

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBK.014

    Plasmid

    #187215

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEXNKI-GST-3C-LIC

    Plasmid

    #108710

    Purpose

    Expression of a GST-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    GST-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-PKM2-G415R

    Plasmid

    #122693

    Purpose

    Express His-tagged PKM2 (G415R) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    G415R

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-Flag-RIPK1-D324A

    Plasmid

    #102678

    Purpose

    retroviral expression plasmid for RIPK1-D324A

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and IRES-GFP

    Expression

    Mammalian

    Mutation

    CHANGE Glutamate 324 to Alanine

    Promoter

    unknown

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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