We narrowed to 15,991 results for: LEA

1. pLIX402 caPHF2

   Plasmid

   #91701

   Purpose

   inducible lentiviral expression of constitutively active PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   caPHF2; S757D, S899D, S954D, S1056D

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pETNKI-strepII-3C-LIC-kan

   Plasmid

   #108706

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PD-L1-miSFIT-4x

   Plasmid

   #124684

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

   Plasmid

   #13617

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 65, Thr 69 and Ser70 to Asp

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pET30a-SRAP-delCys2

   Plasmid

   #84568

   Purpose

   Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   Srap1 (Hmces Mouse)
   

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Mutation

   deletion of Cys2

   Promoter

   T7

   Available Since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1352wUV2 F3bbs kan NNNTGGGCGk

   Plasmid

   #18756

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNHT7::RPL15::sNluc2

   Plasmid

   #186763

   Purpose

   An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-GCaMP3-44

   Plasmid

   #91780

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP3-44
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1352wUV2 F1bbs kan GCGTGGNNN

   Plasmid

   #18753

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pDsRed2-C1-Ahi1

    Plasmid

    #30495

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid

    #70666

    Purpose

    Reduced the expression of gamma-tubulin in murine cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    

    Use

    U6 promoter

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasper-pNos-PCP-eGFP

    Plasmid

    #180274

    Purpose

    to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    PCP-eGFP
    

    Tags

    eGFP

    Expression

    Insect

    Promoter

    nanos

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pg-dsRedXP-HIF-2alpha

    Plasmid

    #87209

    Purpose

    N-terminally dsRedXP-tagged human HIF-2a/EPAS

    Depositor

    Violaine Sée

    Article

    Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

    Insert

    HIF-2a (EPAS1 Human)
    

    Tags

    dsRedXP

    Expression

    Mammalian

    Mutation

    Q868E

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBK.009

    Plasmid

    #187209

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. OVA-miSFIT-2xPerfect

    Plasmid

    #124673

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-Map7-Start-1351

    Plasmid

    #46075

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-Start-1351 (Map7 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. RGG-mCherry-RGG-eDHFR

    Plasmid

    #164648

    Purpose

    Two RGG domains tagged with mCherry and eDHFR

    Depositor

    Huaiying Zhang, Michael Lampson

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    RGG
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI syn iGluf

    Plasmid

    #106121

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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