We narrowed to 15,674 results for: NOL

1. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH-ePPE

   Plasmid

   #183097

   Purpose

   For plant prime editing in rice plants

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. c-Fos

   Plasmid

   #187905

   Purpose

   Expresses c-Fos in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   cFos (FOS Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT120b

   Plasmid

   #122604

   Purpose

   BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   gIII, luxAB, guide RNA
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3X ERE TATA luc

   Plasmid

   #11354

   Purpose

   Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ERE
   

   Use

   Luciferase

   Expression

   Bacterial

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. HPRT

   Plasmid

   #22884

   Depositor

   James Thomson

   Article

   Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

   Insert

   hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
   

   Use

   Cre/Lox; Homologous recombination

   Mutation

   PGK, NeoR, and BGH polyA are between the two geno…

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIRV-NF-kB-eCFP

    Plasmid

    #118094

    Purpose

    Flourescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eCFP
    

    Use

    Retroviral

    Promoter

    minimal promoter with NF-kB responsive element

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xHA-miniTurbo-NLS_pCDNA3

    Plasmid

    #107172

    Purpose

    expresses 3xHA-tagged miniTurbo in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMNb-cALFA

    Plasmid

    #159991

    Purpose

    For the protein expression with ALFA tag at C-terminus in mammalian cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-twin Strep …

    Expression

    Mammalian

    Mutation

    CMV promoter, WPRE

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-puro-DN-hTERT

    Plasmid

    #1775

    Depositor

    Bob Weinberg

    Article

    Hahn et al Nat Med 1999 Oct;5(10):1164-70.

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Dominant negative, 2 base pair mutation, catalyti…

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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