We narrowed to 6,532 results for: cki

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4DdeltaC

Plasmid

#51602

Purpose

expresses Sema4D lacking C terminus

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

Last 69 amino acids of Sema4D C-terminus are dele…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-Neuroserpin oloop

Plasmid

#58260

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
PB-CAG-HRKI(Haunt P-Inr2-cDNA KI)

Plasmid

#92163

Purpose

For lncRNA CAG promoter homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt CAG KI homology arms

Use

Other

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pUASp-Flag-Myc-Qin-E3-attB

Plasmid

#44209

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin (qin Fly)

Use

Fly transformation

Tags

3xFlag and 6xMyc

Mutation

lacking 5xTudor domains on C-term; aa1-274 only

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)

Plasmid

#231349

Purpose

Self complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-PLAP

Plasmid

#222308

Purpose

Synchronize the trafficking of PLAP from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PDE4B-Sensor

Plasmid

#208929

Purpose

in vitro JNK-Sensor for luminescent detection of JNK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLINE1ΔORF2-scFvFc

Plasmid

#206021

Purpose

The ORF2 sequence was removed from pLINE1-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLINE1ΔORF1-2-scFvFc

Plasmid

#206022

Purpose

The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-GFP-T2A

Plasmid

#203608

Purpose

Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHW491

Plasmid

#198808

Purpose

In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::Tetx cDNA::let-858 3'UTR

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_B1ChR2_TS_mScarlet_ER

Plasmid

#195191

Purpose

Highly K+ selective channelrhodopsin

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Bilabrum sp. K+ selective channelrhodopsin 2

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RAPH1Δ536-587

Plasmid

#194865

Purpose

Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

RAPH1 with amino acids 536-587 deleted

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

Plasmid

#191686

Purpose

For knocking p2a-tdTomato into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-tdTomato

Expression

Mammalian

Mutation

WT

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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