We narrowed to 13,186 results for: Mpl;
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Plasmid#127883PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH taggingDepositorInsertGFP-Puro-HBTH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
VPS35 shRNA1
Plasmid#163858PurposeshRNA targeting the coding sequences of VPS35DepositorInsertVPS35 retromer complex component (VPS35 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1QBP
Plasmid#32863PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC1QBP (C1QBP Human)
UseTagsHisExpressionBacterialMutationamino acids E89-Q282 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV10-3xFLAG-prs-MCP1
Plasmid#184341PurposeExpress yeast MCP1 in Expi293F cellsDepositorInsertYeast MCP1 (MCP1 Budding Yeast)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB023f1
Plasmid#99218PurposeComplementary plasmid for aaRS PACE; amber codons in T7 RNAPDepositorInsertPpsp [SD4] T7RNAP(S12*,S203*)
UseTagsExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpspAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-H3FAID
Plasmid#127884PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID taggingDepositorInsertGFP-Puro-H3F-AID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-HF
Plasmid#127882PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F taggingDepositorInsertGFP-Puro-H3F
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTopo pLox hPGK-Puro pA pLox
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 Empty
Plasmid#82446Purpose[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFPDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CP2-TCC
Plasmid#179679PurposeComplementary plasmidDepositorInsert[sd2] T7 RNAP-TCC linker-degron
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-FIP200-MBP
Plasmid#171410PurposeFor the purifcation of human ULK1 complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2.2
Plasmid#134752PurposePCR template for sgRNA cloningDepositorInsertsgRNA-AtU6_29p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2.2
Plasmid#134751PurposePCR template for sgRNA cloningDepositorInsertsgRNA-TaU3p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD554 ZF129x6-Compact_EYFP
Plasmid#138868PurposeEYFP reporter vector for the ZF129x6-Compact promoterDepositorInsertZF129x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF129x6-CompactAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL22Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-CMV-Puro
Plasmid#127169PurposePCR template for CMV-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB023k
Plasmid#99219PurposeComplementary plasmid for aaRS PACE; amber codons in T7 RNAPDepositorInsertPpsp [SD8] T7RNAP(S12*,S203*,S527*)
UseTagsExpressionMutationamber codons at positions 12, 203, and 527 of T7 …PromoterPpspAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP (pJJF001)
Plasmid#173457PurposeC-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1433
Plasmid#129523PurposepDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd GenerationDepositorInsertSpCas9 (Other)
UsePlasmodium, rodent-infectious speciesTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5089
Plasmid#85125PurposeKaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.DepositorInsertPkaiB-kaiB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC18-iSpinach
Plasmid#186053Purposetemplate for co-transcriptional RNA folding assaysDepositorInsertT7A1-rrnG-iSpinach
UseTagsnoExpressionBacterialMutationPromoterT7A1Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD288 ZF43x4-Compact_EYFP
Plasmid#138899PurposeEYFP reporter vector for the ZF43x4-Compact promoterDepositorInsertZF43x4-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x4-CompactAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LasI
Plasmid#193628PurposeInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pISH-OCAM
Plasmid#136767PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Ncam2DepositorInsertNcam2 (Ncam2 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insertPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-AS34a
Plasmid#37099DepositorInsertperfect complementary binding site for hsa-miR-34a (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPD551 ZF92x6-Compact_EYFP
Plasmid#138865PurposeEYFP reporter vector for the ZF92x6-Compact promoterDepositorInsertZF92x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF92x6-CompactAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD289 ZF43x5-Compact_EYFP
Plasmid#138900PurposeEYFP reporter vector for the ZF43x5-Compact promoterDepositorInsertZF43x5-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x5-CompactAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD287 ZF43x3-Compact_EYFP
Plasmid#138898PurposeEYFP reporter vector for the ZF43x3-Compact promoterDepositorInsertZF43x3-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x3-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD286 ZF43x2-Compact_EYFP
Plasmid#138897PurposeEYFP reporter vector for the ZF43x2-Compact promoterDepositorInsertZF43x2-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x2-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD285 ZF43x1-Compact_EYFP
Plasmid#138896PurposeEYFP reporter vector for the ZF43x1-Compact promoterDepositorInsertZF43x1-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x1-CompactAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-GFP-2xmiR-34 (pAW-AS-25)
Plasmid#40833PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-34
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-TRAPPC3
Plasmid#34711DepositorInserttrafficking protein particle complex 3 (TRAPPC3 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPD069 ZF43x6-Spaced_EYFP
Plasmid#138879PurposeEYFP reporter vector for the ZF43x6-Spaced promoterDepositorInsertZF43x6-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-SpacedAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only