We narrowed to 8,487 results for: Set;

1. pSEED-ALFA:HA

   Plasmid

   #219646

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   ALFA:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-sfGFP:ALFA

   Plasmid

   #219642

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVP073

   Plasmid

   #222076

   Purpose

   eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Type

   Empty backbone

   Use

   Chromoprotein-marked pvs1 backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pVP074

   Plasmid

   #222077

   Purpose

   eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Type

   Empty backbone

   Use

   Chromoprotein-marked pvs1 backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pVP075

   Plasmid

   #222078

   Purpose

   AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Type

   Empty backbone

   Use

   Chromoprotein-marked pvs1 backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTmpSm

   Plasmid

   #176228

   Purpose

   A template plasmid for amplification of the cI-hok-strA cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, strA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

   Plasmid

   #211837

   Purpose

   Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVKG1-Puro

    Plasmid

    #108670

    Purpose

    Puromycin resistant cassette for knock-in by VIKING method

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    PURO
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_SUMO_AcCas12n

    Plasmid

    #203808

    Purpose

    pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    SUMO_AcCas12n
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. RP4-11

    Plasmid

    #79812

    Purpose

    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Kanamycin cassette and SacB gene (sacB )
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Km

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pTmpGm

    Plasmid

    #176229

    Purpose

    A template plasmid for amplification of the cI-hok-aacC4 cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, aacC4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCE-P2A-Puro

    Plasmid

    #123318

    Purpose

    Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-UBC-mARG3

    Plasmid

    #173793

    Purpose

    Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpJ, F, G, L, K
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ204-loxP-100RPU.2-ETN-lox2272

    Plasmid

    #154833

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    ETN
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB8644

    Plasmid

    #126919

    Purpose

    Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW071

    Plasmid

    #140037

    Purpose

    PCC 11901 fadD locus KO with a kanamycin resistance cassette

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Kanamycin resistance gene
    

    Use

    Gene knockout in cyanobacteria

    Tags

    Expression

    Mutation

    Promoter

    KanR

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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