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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
IG301: pMVP (L1-R5) CMV promoter

Plasmid

#121692

Purpose

pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-rab5c-CA

Plasmid

#109738

Purpose

Multisite gateway vector for 3' tagging with constitutively active rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Mutation

constitutively active

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab5c

Plasmid

#109719

Purpose

Multisite gateway vector for 3' tagging with rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab2a

Plasmid

#109619

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab2a (rab2a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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miR21mutant-IRE-TREAT

Plasmid

#196931

Purpose

Mutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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miR21-IRE-TREAT

Plasmid

#196930

Purpose

mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7-IRE-TREAT

Plasmid

#196925

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2

Plasmid

#17229

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Tags

His

Expression

Bacterial

Mutation

changed codon 457 to stop codon

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
p3E-rab5aa

Plasmid

#109716

Purpose

Multisite gateway vector for 3' tagging with rab5aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5aa (rab5aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-ccdb-mKate2

Plasmid

#109731

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-mKate2-ccdb

Plasmid

#109732

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLib6.4B

Plasmid

#176665

Purpose

Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MKK4

Plasmid

#29579

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK4 (MAP2K4 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pSicoR human DGCR8-3

Plasmid

#14771

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S11A

Plasmid

#44105

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 11 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only