We narrowed to 17,881 results for: puromycin
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Pooled library#138084PurposeTargets 3' splice sites with paired gRNAs. Induces skipping of poison cassette exons and matching upstream constitutive exons. Compare loss of a constitutive coding exon with loss of a poison exon.DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pLG_GI2
Plasmid#111593PurposemU6-sgRNA GI Library vectorDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG_GI4
Plasmid#111595PurposeGI Library VectorDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLRC1-NEK10p:NEK10-3XFLAG
Plasmid#137030PurposeLentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoterDepositorInsertNIMA-like kinase 10 (NEK10 Human)
UseLentiviralTags3XFLAGPromoterhuman NEK10 endogenous promoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst-mBMP7
Plasmid#127375PurposeAllows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-iRFP670
Plasmid#133397PurposePiggybac vector for constitutive expression of iRFP670-NLSDepositorInsertiRFP-670-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-EGFP
Plasmid#133400PurposePiggybac vector for constitutive expression of EGFPDepositorInsertEGFP
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CreLite
Plasmid#131786PurposeLentiviral vector expressing CreLite, driven by CBh promoterDepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterCBhAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-P112H
Plasmid#133760PurposeLentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged proline 112 to histidine.PromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPC2(N257A)-mCherry
Plasmid#135186PurposePore-dead mutant of TPC2, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of asparagine257 to alanineAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPU-huTRIMmiR30-TRIM5
Plasmid#132938PurposeAll-in-one huTRIM5 rescue-TRIM5 shRNA knockdownDepositorInserthuman TRIM5
UseLentiviral and RNAiExpressionMammalianPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
C1ORF43-GFP
Plasmid#130879PurposeExpresses human C1ORF43 with C-terminal GFP tagDepositorAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 1
Plasmid#131345PurposegRNA targeting mouse TRAF6DepositorAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-CR-PINK1-C-TAP
Plasmid#128510PurposeLentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.DepositorInsertPTEN induced kinase 1 (PINK1 Human)
UseLentiviralTagsFLAG and HA tagsExpressionMammalianMutationSynonymous silent mutation in Q5 to render sgRNA …PromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SETDB1 ts1
Plasmid#115859PurposeSETDB1 knockdownDepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
Plasmid#105039PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-RTP1S-Integration
Plasmid#129458PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses RTP1SDepositorInsertRTP1 (Rtp1 Mouse)
UseSynthetic BiologyExpressionMammalianMutationRemoval of the first 36 amino acids of the proteinPromoterTRE (Tet-On)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68
Plasmid#126548PurposeExpresses FLAG-HA-tagged RNF68 in mammalian cellsDepositorAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgMYC
Plasmid#125770Purposeconstitutive expression of a guide RNA targeting human MYC (CRISPR positive control)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgMYC (MYC Human)
UseCRISPRAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shWRN1
Plasmid#125781Purposeconstitutive expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN1 (WRN Human)
UseRNAiAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only