We narrowed to 23,303 results for: puromycin

1. lentiCRISPR - C9orf114 sgRNA 2

   Plasmid

   #70654

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C9orf114
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-6NBC-GFP-PGK-Puro-Barcode83

   Plasmid

   #83760

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode83
   

   Use

   Retroviral

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBRIT Pax7 N33-170C

   Plasmid

   #17526

   Depositor

   Michael Rudnicki

   Article

   McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

   Insert

   Pax7 (Pax7 Mouse)
   

   Use

   Cre/Lox and Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of aa 34-159 (paired domain)

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBRIT Pax7 N215-273C

   Plasmid

   #17527

   Depositor

   Michael Rudnicki

   Article

   McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

   Insert

   Pax7 (Pax7 Mouse)
   

   Use

   Cre/Lox and Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of aa 216-272 (homeodomain)

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pQCXIP X2/pTER shLUC (w454-1)

   Plasmid

   #17491

   Purpose

   Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase shRNA
   

   Use

   RNAi and Retroviral

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenCMV-PMMars1CyGFP

   Plasmid

   #78315

   Purpose

   Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning protein

   Depositor

   Alan Waggoner

   Article

   Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

   Insert

   Mars1Cy-TMD-GFP
   

   Use

   Lentiviral

   Tags

   HA epitope

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. shCDK6(2)

   Plasmid

   #73553

   Purpose

   shRNA(2) against CDK6

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

8. shCDK6(1)

   Plasmid

   #73552

   Purpose

   shRNA(1) against CDK6

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. CJ40 (let-7 seed mutant perfect site)

   Plasmid

   #17773

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   let-7 seed mutant perfect site (let-7 Fly)
   

   Use

   Luciferase; Drosophila metallothionein gene promo…

   Tags

   renilla luciferase

   Expression

   Insect

   Mutation

   seed mutant

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pMA3200

    Plasmid

    #46855

    Purpose

    A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    ExoY
    

    Use

    Lentiviral

    Tags

    FKBP E31G, R71G, K105E and Myc

    Promoter

    TRE-Tight

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PRAS40 (mouse) shRNA

    Plasmid

    #15672

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 shRNA (Akt1s1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

12. AIRAP 7: AIRAPmyc.pBABEpuro

    Plasmid

    #21797

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    AIRAP (Zfand2a Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-puro-cfRabin8_4

    Plasmid

    #26713

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Rabin8 RNAi
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Mutation

    RNAi to dog Rabin8 alpha isoform.

    Available Since

    Dec. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pBABE puro rat IRS-3

    Plasmid

    #11362

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-3 (Irs3 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pX459V2.0+N497A+Q695A+Q926A

    Plasmid

    #108300

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459V2.0-eSpCas9(1.1)+K810A

    Plasmid

    #108297

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLENTICRISPR sgNFS1

    Plasmid

    #102979

    Purpose

    Cutting NFS1 genomic locus

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    sgNFS1 (NFS1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-6NBC-GFP-PGK-Puro-Barcode49

    Plasmid

    #83726

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode49
    

    Use

    Retroviral

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-6NBC-GFP-PGK-Puro-Barcode42

    Plasmid

    #83719

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode42
    

    Use

    Retroviral

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge