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mRuby-Beta-Catenin-20

Plasmid

#55848

Purpose

Localization: Tight Junctions, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Insert

Beta-Catenin (Ctnnb1 Mouse)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mApple-VASP-5

Plasmid

#54958

Purpose

Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

VASP

Tags

mApple

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
YCplac111-yVps23-mCherry

Plasmid

#45925

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23 (STP22 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

lac

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Mutation

Y39Stop

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLitR-L1

Plasmid

#74683

Purpose

LitR-L1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSL1-LitR-PLitR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-E1

Plasmid

#74677

Purpose

BetI-E1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSE1-BetI-PBetI-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPsrA-R1

Plasmid

#74688

Purpose

PsrA-R1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSR1-PsrA-PPsrA-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-P2

Plasmid

#74686

Purpose

PhlF-P2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP2-PhlF-PPhlF-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-S1

Plasmid

#74691

Purpose

SrpR-S1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS1-SrpR-PSrpR-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pIcaRA-I1

Plasmid

#74682

Purpose

IcaRA-I1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSI1-IcaRA-PIcaR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pQacR-Q2

Plasmid

#74690

Purpose

QacR-Q2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ2-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAmeR-F1

Plasmid

#74675

Purpose

AmeR-F1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSF1-AmeR-PAmeR-YFP

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR L1-13XLexAop2-L4

Plasmid

#41434

Depositor

Steve Stowers

Insert

13XLexAop2

Expression

Bacterial

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pHR hEF1A-BFP-P2A-rtTA-WPRE

Plasmid

#223288

Purpose

A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

BFP-P2A-rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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WasCFP /pQE-30

Plasmid

#79858

Purpose

Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

Insert

WasCFP

Tags

HisTag

Expression

Bacterial

Mutation

Cyan fluorescent protein mCerulean with substitut…

Promoter

T5 promoter / lac operator

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKD068

Plasmid

#136471

Purpose

SSB-GFP IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-GFP (ssb E. coli)

Tags

superfolder GFP

Expression

Bacterial

Mutation

superfolder GFP inserted between Phe148 and Ser149

Promoter

T7 promoter

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 2

Plasmid

#28228

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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