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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Tags

6His

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-MYC-DYK

Plasmid

#115245

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Tags

MYC-DYK

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-matMYC

Plasmid

#115249

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E34: attB4-mCMVp>-attB1r

Plasmid

#162926

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

minimal CMV IE1 promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E07: attB2r-SV40polyA-attB3

Plasmid

#162917

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E24: attB2r-Strep2-attB3

Plasmid

#162913

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C231-E02: attB2r-NanoLuc-attB3

Plasmid

#162901

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E04: attB4-CMV51p>-attB5

Plasmid

#162973

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized human CMV promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E17: attB4-PGKp>-attB5

Plasmid

#162976

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E36: attB4-CMV51p>-attB1r

Plasmid

#162927

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized CMV promoter

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E25: attB2r-mVenus-attB3

Plasmid

#162905

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E34: attB2r-iRFP670-attB3

Plasmid

#162908

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E19: attB4-mPol2p>-attB1r

Plasmid

#162971

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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