We narrowed to 59,538 results for: cloning

1. p2R3z-AtU3b-tRNA-ccdB-gRNA

   Plasmid

   #118390

   Purpose

   Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   AtU3b promoter
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-S2

   Plasmid

   #85211

   Purpose

   Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   T-Sapphire
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGR

   Plasmid

   #46002

   Purpose

   Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

   Depositor

   Christopher Voigt

   Article

   Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEN276

   Plasmid

   #69150

   Purpose

   Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

   Depositor

   Pierre Germon

   Article

   Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

   Insert

   luxCDABE
   

   Use

   Luciferase

   Promoter

   frr

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-NP-B/BRISBANE/60/2008

   Plasmid

   #187921

   Purpose

   Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Influenza B Nucleoprotein
   

   Tags

   UNTAGGED

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Myc-His(-)-HSulf-2

   Plasmid

   #13004

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 (SULF2 Human)
   

   Tags

   6x His and Myc

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRA109

   Plasmid

   #209342

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xP53_RE::FLuc

   Plasmid

   #178316

   Purpose

   P53 Firefly luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   P53 FLuc Reporter
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC3-yeGFP

   Plasmid

   #176181

   Purpose

   yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   yEGFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-minP:luc2

    Plasmid

    #138354

    Purpose

    Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Luciferase
    

    Expression

    Mammalian

    Promoter

    minimal promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pf hPGK mc2 IRES puro

    Plasmid

    #206237

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLARRYv2-EGFP

    Plasmid

    #233208

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHC79

    Plasmid

    #167551

    Purpose

    Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Type

    Empty backbone

    Use

    Cosmid

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid

    #135267

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0M-S-mKOκ-EC18154

    Plasmid

    #137077

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xAP-1_RE::Renilla

    Plasmid

    #178317

    Purpose

    AP-1 Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    AP-1 Renilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

    Plasmid

    #158167

    Purpose

    Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMK1D (CAMK1D Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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