We narrowed to 15,191 results for: invs

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CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-efSaCas9-BlastR

Plasmid

#220971

Purpose

Expresses human codon-optimized efSaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

efSaCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

Plasmid

#217691

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE3 (ADGRE3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-338

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

Plasmid

#217690

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE2 (ADGRE2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-517

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-mitochondria/pcDNA3

Plasmid

#51973

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only
CAG-EnR

Plasmid

#19715

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

engrailed (en Fly)

Expression

Mammalian

Mutation

Drosophila engrailed repressor domain

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8-Magneto2.0

Plasmid

#74333

Purpose

For gateway cloning Magneto2.0 into destination vector

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0

Use

Gateway vector

Tags

Flag

Mutation

TRPV4: delta 760-871

Promoter

none

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4 Smad2

Plasmid

#16484

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad2 (SMAD2 Human)

Tags

Double HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac flag Bmi1

Plasmid

#1967

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Bmi1 (Bmi1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
3xFRB-myc

Plasmid

#20226

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-Mirk (YF)

Plasmid

#17845

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Tags

6xHis and Anti-XPress

Expression

Mammalian

Mutation

Y271/273F

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase30

Plasmid

#59124

Purpose

FAK fret control sensor without FERM domain with 30 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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eTC GFP ß-actin ΔZip

Plasmid

#27124

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

This construct lacks β-actin 3′UTR and therefore …

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNox1

Plasmid

#58340

Purpose

expresses mouse Nox1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

Mus musculus NADPH oxidase 1 alpha (Nox1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag mTOR S2035T R2109A

Plasmid

#26606

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Mutation

S2035T R2109A

Available Since

Nov. 18, 2010

Availability

Academic Institutions and Nonprofits only