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pEBTetDBl-CLIP-Cep76

Plasmid

#136863

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep76 (CEP76 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Cep63

Plasmid

#136861

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep63 (CEP63 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Cep27

Plasmid

#136858

Purpose

Mammalian expression of the centrosomal protein Cep27 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep27 (HAUS2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep76

Plasmid

#136862

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep76 (CEP76 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep63

Plasmid

#136860

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep63 (CEP63 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetBl-CLIP-Cep27

Plasmid

#136857

Purpose

Mammalian expression of the centrosomal protein Cep27 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep27 (HAUS2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetBl-CLIP-Cep97

Plasmid

#136849

Purpose

Mammalian expression of the centrosomal protein Cep97 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep97 (CEP97 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetBl-CLIP-γ-tubulin

Plasmid

#136848

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBG (TUBG1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-α-tubulin

Plasmid

#136840

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Cep70

Plasmid

#136822

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep70 (CEP70 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetD-SNAP-Cep76

Plasmid

#136814

Purpose

Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep76 (CEP76 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-α-tubulin

Plasmid

#136792

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pS6-N-hα2M-H6

Plasmid

#128496

Purpose

Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-A788) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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xCIAR_pcDNA5/FRT/TO

Plasmid

#86504

Purpose

Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

xCIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

F929A and T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hMIB2_#9

Plasmid

#75255

Purpose

retroviral shRNA vector against human MIB2, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against MIB2 (human) (MIB2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSR13

Plasmid

#69155

Purpose

read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
swsn-1 (swsn-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, codon-optimzed index 1.…

Promoter

rps-27 and swsn-1

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLPo-Myc

Plasmid

#124641

Purpose

AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

FLPo

Use

AAV and Cre/Lox

Tags

myc tag

Expression

Mammalian

Promoter

EF1a

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP2B-3x-55-96

Plasmid

#232015

Purpose

Expression of 3 tandem copies of CHMP2B helix 2, connected with gly-ser linkers, attached to sfGFP.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP2B-201-213

Plasmid

#232016

Purpose

Expression of the CHMP2B VPS4-binding region attached to sfGFP.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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