We narrowed to 3,408 results for: guide rna expression plasmid

1. PAJ407

   Plasmid

   #104144

   Purpose

   Expresses 3A_v9 igRNA and sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PAJ426

   Plasmid

   #104145

   Purpose

   Expresses HIV3 igRNA and sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

   Plasmid

   #113159

   Purpose

   An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2_8xCTS-ECFP

   Plasmid

   #200249

   Purpose

   Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   8xCTS-ECFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

   Plasmid

   #113156

   Purpose

   An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat Rosa26
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAN056

    Plasmid

    #220052

    Purpose

    Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR (exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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