We narrowed to 59,538 results for: cloning

1. pDD284

   Plasmid

   #66825

   Purpose

   TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

   Plasmid

   #227775

   Purpose

   Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   BFP-MCS
   mCherry-t2A-CreERT2
   

   Use

   Zebrafish expression

   Promoter

   Olactb and hsp70l

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/Myc-His(-)-MSulf-2

   Plasmid

   #13008

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 2 (Sulf2 Mouse)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Bxb1-GA Donor_CAG

   Plasmid

   #229780

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pL0R_lacZ

   Plasmid

   #202095

   Purpose

   Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pKO (Kan)

   Plasmid

   #110086

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pG-HIV-2LTR

   Plasmid

   #104590

   Purpose

   standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   2LTR circle junction of HIV-1
   

   Use

   Cloning vector

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLARRYv2-EGFP-T2A-Flpo

   Plasmid

   #233212

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCS111

    Plasmid

    #209332

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-NR2F2

    Plasmid

    #138359

    Purpose

    Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Available Since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. CMV-HA-hTAF6

    Plasmid

    #17988

    Depositor

    Naoko Tanese

    Article

    Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

    Insert

    TAF6 (TAF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pKO (Apr)

    Plasmid

    #110088

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO V5 HSPA6

    Plasmid

    #19513

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-secAnxaV-NS

    Plasmid

    #67718

    Purpose

    Gateway entry clone containing secreted human AnxaV with no stop codon

    Depositor

    Rob Parton

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    secAnxaV-NS
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/Myc-His(-)-MSulf-1

    Plasmid

    #13007

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 1 (Sulf1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5-FRT-GFP-mCherry-3pGW

    Plasmid

    #53965

    Purpose

    Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

    Inserts

    GFP
    mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge