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Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLucWm1A
Plasmid#44163DepositorInsertfirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB (CMV enhancer/chicken beta-actin promoter)Available sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT224_(pCA-tTA2)
Plasmid#36430DepositorInserttetracycline transactivator
UseTagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas13d-T2A-GFP(1-10)
Plasmid#224566PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.DepositorInsertCas13d-T2A-GFP(1-10)
UseCRISPRTagsT2A-split GFP(1-10)ExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xPax7gRNA
Plasmid#224569PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xPax7gRNA
Plasmid#224571PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTomatoSlicer
Plasmid#221085PurposeDual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLacZ-LucWm1A
Plasmid#44167DepositorInsertsLacZ
firefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB, CMV enhancer/chicken beta-actin promoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT225_(pCA-tdT3Myc)
Plasmid#36873DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT255_(pCA-GFP4m)
Plasmid#36877DepositorInsertGFP
UseTagsExpressionMammalianMutationF64L; S65T; V163A; S175GPromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT241_(pCA-ATG-intron-tTA2)
Plasmid#36875DepositorInserttTA2
UseTagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT259_(pRosa26-G-tTA2)
Plasmid#36878DepositorInsertsGFP
tTA2
beta-globin intron
Neo
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T_A1aY1
Plasmid#158751PurposeLentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagRFP-T_A1aY1
UseLentiviralTagsTagRFP-TExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 XBB Spike
Plasmid#195287PurposeExpresses codon-optimized full length SARS-CoV-2 XBB SpikeDepositorInsertSARS-CoV-2 XBB Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24-27…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceFeb. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TagBFP_A1aY1
Plasmid#158752PurposeLentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagBFP-T_A1aY1
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only