Showing: 41 - 60 of 76 results
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Plasmid#179514PurposeTunable and temporal expression control of SPI1DepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GS PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86 mIL-15
Plasmid#183245PurposeExpression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsert41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralTagsExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R2
Plasmid#61383PurposeExpresses TRAIL receptor 2DepositorInsertTRAIL receptor 2 (TNFRSF10B Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pD649-HAsp-TNFRSF10B-COMP5AP-AviTag-9xHis
Plasmid#157111PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF10B (TNFRSF10B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
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pD649-HAsp-TNFRSF10B-Fc(DAPA)-AviTag-6xHis
Plasmid#156546PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF10B (TNFRSF10B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bik-s2193
Plasmid#177425PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-L61G-pEGFP-C1
Plasmid#177426PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationLeucine 61 replaced with glycinePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-GG153AA-pEGFP-C1
Plasmid#177427PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycines 153 and 154 mutated to alaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-G154STOP-pEGFP-C1
Plasmid#177428PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycine 154 replaced with STOP codonPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-cb5-pEGFP-C1
Plasmid#177431PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationBik MBR removed (after position 136) and replaced…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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Venus-Bik-DD-pEGFP-C1
Plasmid#177429PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationThreonine 33 and Serine 35 replaced with aspartic…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-AA-pEGFP-C1
Plasmid#177430PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationThreonine 33 and Serine 35 replaced with alaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-delMBR-pEGFP-C1
Plasmid#177432PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationBik MBR removed (after position 136)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 76 results