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We narrowed to 238 results for: LbCas12a

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Showing: 41 - 60 of 238 results

  3. pCAG-LbCas12a-Plus-mCherry

    Plasmid
    #191230
    Purpose
    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    D156R, R883K, R887A
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191230-plasmid-map-sequence-id-404705

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  4. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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  6. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155046-plasmid-map-sequence-id-308670

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  7. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114070-plasmid-map-sequence-id-220511

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  10. pYPQ230-sbcB-N

    Plasmid
    #220290
    Purpose
    Gateway entry plasmid (attL1& attR5) expressing sbcB exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter
    Depositor
    Yiping Qi
    Publication
    Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50
    Insert
    sbcB-XTEN linker-LbCas12a
    Use
    CRISPR; Gateway compatible sbcb-xten linker- lbca…
    Expression
    Plant
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    220290-plasmid-map-sequence-id-436164

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  12. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid
    #160575
    Purpose
    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.
    Depositor
    Diego Orzaez
    Publication
    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AtU6-26:LbCas12a_DR
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160575-plasmid-map-sequence-id-322087

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  13. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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  15. pYPQAT43

    Plasmid
    #223415
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR; T-dna vector
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223415-plasmid-map-sequence-id-453575

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  16. pYPQAT45

    Plasmid
    #223417
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223417-plasmid-map-sequence-id-453515

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  18. pYPQAT44

    Plasmid
    #223416
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223416-plasmid-map-sequence-id-453533

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  20. pYPQAT49

    Plasmid
    #223421
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223421-plasmid-map-sequence-id-454169

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Showing: 41 - 60 of 238 results