We narrowed to 398 results for: pina

1. Mouse genome-wide lentiviral CRISPR gRNA library version 2

   Pooled Library

   #67988

   Purpose

   Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Species

   Homo sapiens, Mus musculus, and Synthetic

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pTP-AAV-ChATen-mCherry

   Plasmid

   #193741

   Purpose

   This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

   Depositor

   Hynek Wichterle

   Insert

   ChAT enhancer, mini promoter, synthetic intron, mCherry
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   1000 bp ChAT enhancer with a 32 bp minimal promot…

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-CAGIG-H2B-V5-mScarlet

   Plasmid

   #191093

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B and mScarlet
   

   Use

   AAV

   Tags

   H2B-V5-mScarlet

   Expression

   Mammalian

   Mutation

   The fusion protein was optimized to the Human cod…

   Promoter

   CMV and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV2-CAG-3xNLS-vsfGFP-0

   Plasmid

   #191097

   Purpose

   To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-vsfGFP-0
   

   Use

   AAV

   Tags

   Green fluorescent protein bound to enhancer nanob…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-CAG-3xNLS-AausFP1

   Plasmid

   #191096

   Purpose

   To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-AausFP1
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Gregory Petsko, Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV2-CAG-mScarlet-I-Cdt1

   Plasmid

   #191100

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mScarlet-I-Cdt1 (30-120)
   

   Use

   AAV and Synthetic Biology

   Tags

   mScarlet-I fused to residues 30-120 of human Cdt1…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK275

    Plasmid

    #73363

    Purpose

    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

    Depositor

    T. J. Kappock

    Insert

    delta-8 sphingolipid desaturase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    missing residues 1-6

    Promoter

    GAL1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK369

    Plasmid

    #73594

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    FLAG tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK137

    Plasmid

    #73313

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    5'-AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK108

    Plasmid

    #73312

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK141

    Plasmid

    #73316

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6, Pas2p signal sequence, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK276

    Plasmid

    #73366

    Purpose

    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)

    Depositor

    T. J. Kappock

    Insert

    plastid omega-3 fatty acid desaturase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutation I231F. Also insert is missing residues 1…

    Promoter

    GAL1

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK142

    Plasmid

    #73317

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6, Pas2p, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene. Encodes a glycine-204 to serine m…

    Promoter

    AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK370

    Plasmid

    #73595

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Silent mutation in Arg299 codon.

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-LAP2-YFP-NLS

    Plasmid

    #25847

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LAP2-YFP-NLS
    

    Use

    Tags

    His and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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